Table 3.

Wall staining properties of root hairs and cells of outer, middle and inner cortex in fresh sections of roots of L. barbata. Observations were made from each of 10–20 roots.

Distance from tip (mm)	Stain*	Root hair (rh), epidermis (ep)	Outer cortex	Middle cortex	Inner cortex	Figure
∼10	TB	Pink/red	Blue	Blue	Blue	Fig. 6A
	RdB†	Light orange	–	–	–	Fig. 6D
	PG	rh base faint, ep +	–	–	–	Fig. 6H
∼20	TB	Blue/green	Blue/green	Blue/green	Blue/green	Fig. 6B
	RdB	rh and ep bright orange	–	–	–	Fig. 6E
	PG	rh base faint, ep +	Ni	Ni	Ni	Ni
∼100	TB	rh blue, ep blue/brown	Brown/blue	Greenish	Dark blue	Fig. 6C
	NS		Dark brown			Ni
	RdB	Orange/blue	–	–	–	Fig. 6F
	PG	+++	faint	–	+++	Fig. 6I
∼180	TB	Dark green	Dark brown	Brown	Dark green	Ni
	NS	Grey/brown	Brown	Light brown	Brown	Ni
	RdB	rh, orange outer layer	–	–	–	Fig. 6G
	PG	+++	+++	–	+++	Ni

Ni = not illustrated.

* TB, Toluidine blue; RdB, Rhodamine B-induced fluorescence; PG, Phloroglucinol/HCl; NS, not stained, brown endogenous pigment. + = Positive reaction; intensity of staining (+) clear positive staining to (+++) intense positive staining. − = no staining reaction/colours.

^† Rhodamine B outer, middle, inner cortex (–). We record only the yellow to bright orange fluorescence induced by this fluorochrome, which is known to indicate lipidic material (see text). Other colours in the sections result from underlying autofluorescence, non-fluorescent staining and endogenous pigment which may or may not have masked a positive reaction.