Figure 2.

Unfolded protein response in palmitate-exposed human islets. The islets were cultured for 3, 24 or 48 hours in the presence of 0.5 mM palmitate (Pa) at 5.5 or 25 mM glucose (Glu). AICAR (1 mM, Aic) or etomoxir (0.2 mM, Et) were added as indicated. Islets cultured at 5.5 mM glucose alone were considered as control. After culture, protein levels of p-eIF2α (A), CHOP (B) and BiP (C) were measured. Protein levels are means ± SEM of 4 independent experiments.*P < 0.05 compared to control.