Figure 7. Combined treatment started at day 8 is not effective to eliminate B!6 melanoma.

A. In vivo experiment. Mice were subcutaneously implanted with B16-OVA cells on day 0 and treated on day 8 with 100 µg of rat IgG (control) or anti-CD137 mAb i.p. Mice also received on the same day activated or resting OT-1 cells i.v. B. In silico experiment. B16-OVA cells were injected toward the immediate neighborhood in the center of lattice at timestep 0. Immunotherapy on day 8 with 100 µg of rat IgG (control) or anti-CD137 mAb i.p. were simulated injecting the compound around the lattice walls. Virtual mice that also received in the same day activated or resting OT-1 T cells i.v. were simulated in the same way.