Table 1. Total energy of before and after mutation residue on β2 Chain B.

Name	Amino acids	PositionChromosome11	Before point mutation Energy E/KT units	After point mutation Energy E/KT units	Before Total protein EnergyE/KT units	After Total protein EnergyE/KT units
HbC	Glu→Lys (E→K)	6	−3.734	−3.033	−612	−608
HbD	Glu→Gln (E→Q)	121	−6.621	−1.980	−612	−607
HbE	Glu→Lys (E→K)	26	−2.100	1.611	−612	−575
HbS	Glu→Val (E→V)	6	−3.734	−2.825	−612	−607

Where “E” is the energy, “−E/KT” is the frequency that is proportional to exp, “T” is the temperature and “K” is the Boltzmann constant.