Table 3. RMSD and total energy of native structure (4HHB) and mutant modeled structures.

Name	Aminoacid variant	RMSD native and mutant protein structure	Total energy after minimization KJ/mol
HbC	Glu→Lys (E→K)	0.50	−334383.20
HbD	Glu→Gln (E→Q)	0.42	−337822.06
HbE	Glu→Lys (E→K)	0.35	−336831.04
HbS	Glu→Val (E→V)	0.25	−333364.02

The total energy of native structure (4HHB) after energy minimization is −334380.5 kJ/mol (score: 0.69) and before energy minimization it was 29644578.1 kJ/mol (score: −3.31).