Figure 1. Global density variation SV analysis of the phosphorylase B sample recorded with the absorbance data at 280 nm.

The sets of panels present the data in (A) H₂O, (B) 50% H₂ ¹⁸O, and (C) 90% H₂ ¹⁸O based buffer. For each set of panels, the measured data (corrected for the time-invariant noise contributions) are shown as solid lines, and the global best-fit profiles are shown as thin dotted lines (virtually superimposed to the data). Higher color temperatures indicate later times. Below are the residuals bitmap (a 2d grey-scale representation of residual values with time plotted vertically and radius horizontally [69]) and the residuals of the fit, with rmsd of 0.00365 OD (A), 0.00411 OD (B), and 0.00380 (C). In the presence of H₂ ¹⁸O, fewer scans were included into the analysis in order to achieve similar numbers of total scans representing the sedimentation process. The best-fit c(s) distribution from this analysis is shown in Figure 2.