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. 2011 Oct 20;6(10):e26018. doi: 10.1371/journal.pone.0026018

Figure 4. VVH does not co-localize with three major lipid raft molecules or a non-lipid raft molecule.

Figure 4

CHO cells were fixed, and then incubated with 5 µg/ml VVH. After washing the cells with PBS, the cells were incubated with anti-VVH and biotin- conjugated CTxB, anti-cav-1, anti-flt-1 or anti-TfR. The cells were probed with Alexa 488-conjugated anti-rabbit anti-body (for VVH) and Alexa 546-conjugated streptavidin (for CTxB) or Alexa 546-conjugated anti-mouse anti-body (for cav-1 and flt-1). Images were obtained by FW4000 fluorescent microscopy.