Skip to main content
. 2011 Oct 20;6(10):e26018. doi: 10.1371/journal.pone.0026018

Figure 5. Cholesterol sequestering did not affect VVH cytotoxicity in most cell lines.

Figure 5

CHO (A), J774A.1 (B), Caco-2 (C) and HeLa cells (D) were incubated with (+) or without (−) 8 mM MβCD at 37°C for 1 h. After washing the cells, the cells were incubated with 1 µg/ml of VVH (open bar) for 3 h or 50 HU/ml of SLO (closed bar) for 1.5 h at 37°C. The cytotoxicity in these cells was assayed by the release of lactate dehydrogenase (LDH). Data are represented as the mean ± SD and represent more than three independent experiments, each in triplicate wells. *, analysis of variance and Tukey's test, P<0.01.