Table 4. Median fluorescence intensity (MFI) values reflecting antigen-specific IgG and IgA levels in rhesus macaques (RM) and healthy human volunteers (HV).
| MFI value, median (range) | MFI value, median (range) | ||||||
| S. aureus protein (antibody isotype) | RM | HV | P a | S. aureus protein (antibody isotype) | RM | HV | P a |
| alpha toxin (IgG) | 14337 (7438–15574) | 14587 (6355–17465) | 0.285 | SEA (IgG) | 88 (17–3565) | 3839 (29–11365) | <0.001 |
| alpha toxin (IgA) | 1540 (139–4729 | 1775 (215–9549) | 0.154 | SEA (IgA) | 16 (1–449) | 247 (5–9345) | <0.001 |
| CHIPS (IgG) | 20 (0–2580) | 13310 (2933–16281) | <0.001 | SEB (IgG) | 34 (0–2836) | 4223 (190–13622) | <0.001 |
| CHIPS (IgA) | 1 (0–130) | 7494 (957–11238) | <0.001 | SEB (IgA) | 2 (0–673) | 90 (2–398) | <0.001 |
| ClfA (IgG) | 454 (16–8250) | 4029 (670–13449) | <0.001 | SEC (IgG) | 4551 (1–14006) | 12720 (4713–15884) | <0.001 |
| ClfA (IgA) | 580 (4–6237) | 673 (145–4730) | 0.373 | SEC (IgA) | 280 (0–4896) | 600 (63–3943) | 0.004 |
| ClfB (IgG) | 1066 (65–7518) | 7272 (2499–14127) | <0.001 | SED (IgG) | 84 (3–533) | 800 (33–7924) | <0.001 |
| ClfB (IgA) | 1232 (0–6962) | 2357 (255–10038) | 0.125 | SED (IgA) | 1 (0–85) | 19 (0–736) | <0.001 |
| Efb (IgG) | 622 (162–6325) | 4238 (770–12855) | <0.001 | SEE (IgG) | 3 (0–229) | 258 (7–3017) | <0.001 |
| Efb (IgA) | 69 (0–1575) | 4775 (257–8125) | <0.001 | SEE (IgA) | 0 (0–151) | 12 (0–1856) | <0.001 |
| ETA (IgG) | 156 (10–5670) | 234 (16–14891) | 0.217 | SEG (IgG) | 37 (0–3861) | 517 (5–4385) | 0.002 |
| ETA (IgA) | 30 (1–366) | 19 (6–1127) | 0.742 | SEG (IgA) | 5 (0–353) | 22 (0–432) | 0.035 |
| ETB (IgG) | 72 (0–10405) | 55 (1–2855) | 0.515 | SEH (IgG) | 1282 (0–10804) | 1400 (9–15602) | 0.468 |
| ETB (IgA) | 3 (0–1023) | 20 (1–1529) | 0.026 | SEH (IgA) | 88 (0–2083) | 44 (0–2158) | 0.821 |
| FnbpA (IgG) | 208 (20–3374) | 1269 (222–6132) | <0.001 | SEI (IgG) | 4 (0–5154) | 257 (0–3544) | <0.001 |
| FnbpA (IgA) | 73 (1–1047) | 744 (26–5261) | <0.001 | SEI (IgA) | 0 (0–111) | 8 (0–840) | <0.001 |
| FnbpB (IgG) | 111 (9–1089) | 478 (54–2758) | <0.001 | SEJ (IgG) | 105 (5–7274) | 2538 (95–16246) | <0.001 |
| FnbpB (IgA) | 15 (2–253) | 253 (19–2603) | <0.001 | SEJ (IgA) | 1 (0–217) | 25 (0–2236) | <0.001 |
| HlgB (IgG) | 8519 (2595–10365) | 10761 (2979–13212) | 0.001 | SEM (IgG) | 12 (0–7325) | 307 (8–2738) | <0.001 |
| HlgB (IgA) | 474 (10–2799) | 556 (44–6394) | 0.233 | SEM (IgA) | 1 (0–354) | 56 (0–1128) | <0.001 |
| IsdA (IgG) | 2811 (508–8602) | 2665 (576–5183) | 0.732 | SEN (IgG) | 7 (0–1166) | 631 (0–2672) | <0.001 |
| IsdA (IgA) | 248 (13–3997) | 357 (9–8058) | 0.940 | SEN (IgA) | 0 (0–155) | 7 (0–2743) | <0.001 |
| IsdH (IgG) | 929 (44–8170) | 3921 (605–9420) | <0.001 | SEO (IgG) | 40 (7–2009) | 306 (147–653) | <0.001 |
| IsdH (IgA) | 311 (3–4328) | 294 (43–2117) | 0.661 | SEO (IgA) | 3 (0–117) | 20 (4–544) | <0.001 |
| LukD (IgG) | 1082 (1852–15031) | 12226 (3781–16731) | 0.011 | SEQ (IgG) | 5 (0–7125) | 341 (0–3763) | <0.001 |
| LukD (IgA) | 641 (0–3418) | 694 (106–4088) | 0.753 | SEQ (IgA) | 0 (0–132) | 19 (0–2219) | <0.001 |
| LukE (IgG) | 12386 (5270–14191) | 12541 (5955–15590) | 0.732 | SER (IgG) | 3 (0–1797) | 63 (0–2654) | <0.001 |
| LukE (IgA) | 1181 (28–5431) | 664 (38–7766) | 0.524 | SER (IgA) | 0 (0–40) | 9 (0–357) | 0.001 |
| LukF (IgG) | 7321 (1240–12631) | 10579 (2984–14996) | 0.007 | SSL1 (IgG) | 8783 (1520–14022) | 4683 (518–11914) | 0.005 |
| LukF (IgA) | 321 (0–2554) | 433 (38–3916) | 0.041 | SSL1 (IgA) | 198 (0–4066) | 195 (6–2947) | 0.956 |
| LukS (IgG) | 10470 (1939–14385) | 8734 (1662–16976) | 0.135 | SSL11 (IgG) | 867 (43–8824) | 1107 (122–8955) | 0.311 |
| LukS (IgA) | 602 (0–6321) | 1015 (13–10014) | 0.524 | SSL11 (IgA) | 46 (3–1495) | 96 (8–2162) | 0.215 |
| SasG (IgG) | 89 (3–3376) | 197 (3–2791) | 0.093 | SSL3 (IgG) | 7280 (898–12949) | 7813 (1618–15432) | 0.403 |
| SasG (IgA) | 8 (0–5408) | 33 (4–3950) | 0.009 | SSL3 (IgA) | 321 (5–3062) | 519 (24–5234) | 0.244 |
| SCIN (IgG) | 187 (5–7259) | 11333 (7572–15438) | <0.001 | SSL5 (IgG) | 4138 (621–9547) | 3267 (511–5928) | 0.021 |
| SCIN (IgA) | 14 (0–1220) | 2945 (211–10036) | <0.001 | SSL5 (IgA) | 97 (6–1293) | 115 (27–933) | 0.264 |
| SdrD (IgG) | 233 (17–2844) | 1683 (400–6924) | <0.001 | SSL9 (IgG) | 11968 (4788–13605) | 9255 (1005–15161) | 0.150 |
| SdrD (IgA) | 64 (3–2205) | 634 (48–4758) | <0.001 | SSL9 (IgA) | 444 (35–3523) | 138 (20–1158) | 0.001 |
| SdrE (IgG) | 250 (28–6655) | 2045 (251–8626) | <0.001 | TSST-1 (IgG) | 32 (4–9350) | 12051 (0–16927) | <0.001 |
| SdrE (IgA) | 218 (0–3017) | 604 (28–3650) | 0.015 | TSST-1 (IgA) | 4 (0–125) | 932 (10–3504) | <0.001 |
a
Differences in antigen-specific MFI values between groups were considered to be statistically significant at P<0.05 (Mann-Whitney U test).