Figure 4. Stress response proteins are differentially expressed in rSARS-CoV-ΔE versus rSARS-CoV-infected cells.
Total protein from Vero E6 (A) and MA104 (B) cells infected with rSARS-CoV-ΔE or SARS-CoV was extracted at 22 and 75 hpi, respectively. Levels of hsp90 and hsp60 were normalized to those of GAPDH after Western-blot assay (left panels) and densitometric analysis of the bands (right panels). Columns represent hsp90/GAPDH and hsp60/GAPDH ratios in SARS-CoV-ΔE (red) or SARS-CoV (blue) infected cells. Error bars indicate the standard deviation from three independent experiments.
