Table 2. Gene expression in FLS after stimulation with IL-17A.
| mRNA copies/β2 microglobulin×100 | |||
| Control | IL-17A | p-value | |
| IL-1β | n.d. | n.d. | - |
| IL-6 | 0.065±0.01 | 2.1±0.3 | 0.003 |
| KC/CXCL1 | 6.4±0.6 | 80.4±18.1 | 0.015 |
| MIP-2/CXCL2 | 0.14±0.03 | 0.33±0.09 | 0.102 |
| LIX/CXCL5 | 27.8±1.7 | 641.9±123.5 | 0.008 |
| MIP-1γ/CCL9 | 1.1±0.1 | 2.0±0.2 | 0.015 |
| MIP-3α/CCL20 | 0.02±0.004 | 0.74±0.16 | 0.011 |
| MIP-1α/CCL3 | 0.17±0.02 | 0.14±0.03 | 0.360 |
| MIP-3β/CCL19 | 0.44±0.05 | 0. 80±0.13 | 0.059 |
| MCP-3/CCL7 | 40.7±4.4 | 252.4±49.5 | 0.013 |
| RANTES/CCL5 | 0. 32±0.086 | 0.60±0.09 | 0.091 |
| RANKL | 1.37±0.05 | 7.4±1.1 | 0.006 |
| IL-33 | 1.9±0.14 | 3.15±0.48 | 0.068 |
| VEGF-A | 20.5±1.4 | 24.0±5.3 | 0.553 |
| MMP2 | 277.3±38.4 | 271.6±41.4 | 0.924 |
| MMP3 | 115.3±16.9 | 339.5±45.0 | 0.010 |
| MMP13 | 103.3±3.3 | 249.2±71.3 | 0.110 |
Genes induced in FLS by IL-17A. FLS were left unstimulated or stimulated with 100 ng/ml IL-17A. After 16 h, total RNA was harvested and gene expression was determined by qPCR. Data shown represent the 100-fold of the mean of mRNA copies/β2 microglobulin mRNA copies ± SEM (n = 3 independent experiments). Differences were evaluated by unpaired two-tailed Student's t test; p<0.05 was considered statistically significant and is indicated in bold.