Table 1. Antibodies used for immunohistochemistry.
| Target antigen | Species/isotype | Working dilution | Incubation time/Temperature | Antigen demasking* | Source |
| Proteolipid Protein (PLP) | Mouse IgG2a | 1∶1000 | 24 h/4°C | Citrate | Serotec, Oxford (UK) |
| Neurite Outgrowth Inhibitor Protein A (NOGO-A) | Rabbit polyclonal | 1∶1000 | 1 h/RT | Citrate | Chemicon, Temecula (CA), USA |
| MAC-3 | Rat IgG1, K | 1∶200 | 24 h/4°C | Citrate | BD Biosciences, Franklin Lakes (NJ), USA |
| CD3 | Rabbit polyclonal | 1∶4000 | 1 h/RT | Tris-EDTA | Sigma-Aldrich, St. Louis (MO), USA |
RT = room temperature.
*Demasking by boiling (micorowaving) sections in the respective buffer for 15 min.