Figure 1. A simplified replication timing fingerprint.

A. Four 200 kb regions in chromosome 7, highlighted in grey, are selected for a simplified fingerprint using two replicates each of ESCs (light and dark blue) and NPCs (light and dark green). B. The replication timing ratio for each region in each experiment is shown, with the total distances in replication timing for all fingerprinting regions between replicates of ESCs or NPCs in grey. Note that distances between the two different cell types (ESC vs. NPC) are substantially higher than those between replicate profiles (e.g., 6.1 for ESC2 vs. NPC1; shown between the grey boxes). C. Total differences in replication timing for all four fingerprinting regions between all combinations of the two replicates from these two cell types are shown. Highlighted in grey are the values for the two replicates of each cell type, which are considerably less than the values for any of the inter-cell type comparisons. Shown below the table is the “Distance ratio”, calculated as the average distance between cell types (or between replicates) divided by the average distance within cell types. The Distance ratio represents the degree of separation between replication profiles in regions used for classification.