Figure 4. Identification of cell type- and pluripotency-specific regions.

A. Construction of a general classifier for distinguishing pluripotent from committed mouse and human cell types, with results summarized in the tables below for the standard kNN method and leave-one-out crossvalidation. B. Representative fingerprint regions are shown for three cases: general classification (left), distinguishing pluiripotent vs. committed cell types (middle), and identifying cell-type-specific (here, lymphoblast-specific) regions (right). Lines represent averaged profiles for each cell type. Several EtoL regions in the pluripotency fingerprint contain genes known to function in maintaining stem cell identity, such as Dickkopf homolog DKK1, while uniquely early regions in cell type-specific fingerprints often feature genes with relevant functional or disease associations, such as IKZF1 in lymphoblast cells.