Table 3. Sequences and restriction sites of PCR primers.

Primer and purpose	Sequence (5′→3′)	Modification
Construction for sequence and YTHS
RACK-F	GCcatatgATGTCGGAAACTTTTGAT	Nde I
RACK-R	CAgaattcTTATCGTGAGACAGCAAC	EcoR I
RPL5-F	CGcatatgATGGGTTTCGTCAAAG	Nde I
RPL5-R	TAgaattcTCACGATTCGGCGTCG	EcoR I
GAPDH3-F	CGcatatgATGTCAAACATTGGAAT	Nde I
GAPDH3-R	GCggatccTTAATCTTTGGTTTGGAT	BamH I
RPL7a-F	GGcatatgATGGTGCAGAAGAAGC	Nde I
RPL7a-R	ATggatccCTAGCCCTGTTTCTGG	BamH I
RPL8-F	ACgaattcATGGGTAGGGTTATCCGCG	EcoR I
RPL8-R	CAggatccCTAATCGTCTCCCTTCTT	BamH I
NCP-F	CGcatatgATGGGTACCAACAAGCC	Nde I
NCP-R	CTggatccCTAGTCATCTGCACCTT	BamH I
Construction of expression vectors
RACK-EcoR-F	CGgaattcATGTCGGAAACTTTTGAT	EcoR I
RACK-Hin-R	TGaagcttTTATCGTGAGACAGCAAC	Hind III
RPL5-Bam-F	CGggatccATGGGTTTCGTCAAAGT	BamH I
RPL5-R	TAgaattcTCACGATTCGGCGTCG	EcoR I
GAPDH3-Bam-F	GCggatccATGTCAAACATTGGAATT	BamH I
GAPDH3-Hin-R	CGaagcttTTAATCTTTGGTTTGG	Hind III
RPL7a-Bam-F	ACggatccATGGTGCAGAAGAAGCC	BamH I
RPL7a-Hin-R	CGaagcttCTAGCCCTGTTTCTG	Hind III
RPL8-F	ACgaattcATGGGTAGGGTTATCCGCG	EcoR I
RPL8-Hin-R	GCaagcttCTAATCGTCTCCCTT	Hind III

Lowercase indicates a restriction enzyme site.