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. 2011 Oct 20;6(10):e26534. doi: 10.1371/journal.pone.0026534

Figure 3. ARMS assay for JAK2 V617F.

Figure 3

Of tracks for each sample, bands in 229-bp suggested the existence of the wild type allele, while a mutant allele was indicated by the presence of a band in 279-bp. The 463-bp product served as a control of amplification. M, 2000 bp DNA ladder; 1, dd H2O water control; 2, HEL cell line DNA as JAK2 V617F homozygous control; 3, RPMI82264 cell line DNA as wild type homozygous control; 4, Patient sample with no JAK2 V617F mutation; 5, Patient sample harboring wild type/JAK2 V617F positive heterozygote.