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. 2011 Oct 20;6(10):e26514. doi: 10.1371/journal.pone.0026514

Figure 5. Treatment with a TGF-beta inhibitor reduces the migratory response to Snail and Slug.

Figure 5

(A) Cell migration assay using MDA-MB-231, uninfected MCF-7 cells, and MCF-7 cells infected with control, Snail or Slug adenoviruses (white bars), treated with DMSO (light grey bars) or with 10 µM final of the TGF-beta inhibitors LY364947 (dark grey bars) or SB431542 (black bars) for 2 days. The X-axis represents the total number of cells in 10 fields. The data represents the average of three independent biological replicates (* T-test p-value ≤0.05, **T-test p-value ≤0.01). (B) Addition of TGF-beta inhibitor does not affect repression of cell junction molecules CDH1, DSP and CLDN4. MCF-7 cells were untreated or treated with control or Snail adenovirus, and with DMSO vehicle or SB431542 for 2 days. RNA was isolated and RT-PCR of cDNA with real-time quantitation was performed following normalization to GAPDH, and MCF-7 Day 0. The data represents the average of three independent biological replicates.