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. 2011 Oct 20;7(10):e1002317. doi: 10.1371/journal.pgen.1002317

Figure 6. Experimental validation of an ultra-edited RNAs.

Figure 6

We experimentally validated ultra-editing in the ESTs DA098819 (A) and DA364252 (B). We generated cDNA from cerebellum RNA and amplified cDNA fragments that correspond to chr19:53120654–53121052 (A) and chr2:242643522–242644012 (B). We cloned the PCR products, sequenced the clones (14 in (A), 13 in (B)), and aligned the sequences to the genomic DNA. In the figure, we show the number of clones with each given number of editing sites. The red, striped bar in each panel indicates the number of sites in the EST. Almost all clones are highly edited, with at least one clone edited to about the same extent as the ultra-edited EST.