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. 2011 Oct 20;6(10):e26601. doi: 10.1371/journal.pone.0026601

Figure 3. Specificity of miR-101 and miR-494 CFTR suppression by recognition of the seed sequence.

Figure 3

(A) Schematic representation of the reporter constructs carrying CFTR 3’UTR mutated at miR-101 responsive element or mir-494 seed sequence. (B) Relative luciferase activity in HEK293 cells over-expressing the indicated miRNAs and transfected with the CFTR wild-type 3’UTR vector or its mutant derivative pCFTR-3’UTR-mut101 and pCFTR-3’UTR-mut494. Data are presented as the normalized activity of miR-transfected cells (miR-101 or miR-494) relative to cells transfected with miR-Ctr. All data are average values ± SE from at least three experiments (ANOVA, ns, not significant; *, p<0.05; ***, p<0.001 compared to the basal value with miR-Ctr).