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. 2007 Feb 7;446(7131):92–96. doi: 10.1038/nature05539

Figure 4. Soluble human TfR1, but not transferrin or human TfR2, modulates New World arenavirus infection.

Figure 4

a, CHO cells (top) and BHK cells (bottom) were transfected with plasmids expressing human TfR1 (dark grey bars) or TfR2 (light grey bars) or with vector alone (white bars), and incubated with the indicated pseudoviruses. Entry was measured as in Fig. 2a. b, 293T cells were incubated for 1 h with MACV pseudovirus produced in serum-free medium and with the indicated amounts of human apo-transferrin (white circles) or holo-transferrin (grey circles) and then washed; the infection level was assessed two days later. c, 293T cells were infected for 1 h with LASV (triangles), MACV (squares) or JUNV (circles) preincubated with increasing concentrations of soluble human TfR1 for 30 min. Cells were washed, and GFP fluorescence was measured two days later. Error bars indicate standard deviation.