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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Anal Chem. 2011 Sep 2;83(19):7400–7407. doi: 10.1021/ac201403y

Figure 2.

Figure 2

Incubation of 1 µg of H3K9Ac with buffer alone (Tris [50 mM, pH 8.0] containing 150 mM NaCl, 0.2 mM NAD and 1mM DTT at 37°C for 4 hours, SIRT-6 CT, H133Y SIRT-6 (CT)-MB and heat inactivated SIRT-6 (CT)-MB.