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. Author manuscript; available in PMC: 2011 Oct 20.
Published in final edited form as: Dev Biol. 2010 Feb 26;341(1):196–204. doi: 10.1016/j.ydbio.2010.02.028

Fig. 4. Temporal development stages when Hh signaling affects artery-vein specification.

Fig. 4

(A–D) Dorsal views displaying comparable levels of flk1-positive angioblasts in wild-type embryos (A, C) and smo mutants (B, D). (E, F) Confocal microscopy analysis revealing the normal size of the dorsal aorta, the posterior cardinal vein and intersomitic vessels in control embryos (E), and an expanded posterior cardinal vein in cyclopamine-treated embryos (F). (G) Schematic representation depicting the temporal activity of cyclopamine in artery-vein specification. Embryos were incubated in 50 μM cyclopamine during a series of developmental window. Red and blue line: artery and vein. Blue line: vein. 5s:5-somite stage. 10s:10-somite stage.