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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Microb Pathog. 2011 Aug 29;51(6):432–441. doi: 10.1016/j.micpath.2011.08.003

Fig. 1.

Fig. 1

Phagocytosis, killing efficiency, and production of highly reactive oxygen species (hROS) by primary peritoneal macrophages isolated from WT mice versus NOX2-/- mice infected with spherule initials of C. posadasii. (A) Phagocytic indices were determined for IFN-γ+LPS-activated macrophages isolated from WT mice (open bars) versus NOX2-/- mice (black bars) and co-cultured with spherule initials using a MOI of 2:1 (macrophages:fungal cells). No statistically significant difference was determined. (B) Fungicidal assays were conducted using monolayers of non-activated, control (Ctl.) macrophages (open bars) versus activated macrophages (black bars) derived from WT or NOX2-/- mice. Estimates of killing efficiency by macrophages were based upon recovered numbers of viable spherule initials which had interacted with (adhered to or engulfed by) the phagocytes. No statistically significant difference was evident between CFUs. (C) hROS production by non-activated (open bars) versus activated macophages (black bars) isolated from non-infected or Coccidioides-infected WT or NOX2-/-mouse strains was measured using a fluorescent probe (APF) and expressed as relative fluorescence units (RFU). A statistically significant difference between hROS production by activated, non-infected or infected WT macrophages versus non-activated, non-infected or infected WT macrophages (*p< 0.001), and between activated WT macrophages versus NOX2-/--derived macrophages (*p<0.0001) were determined. Error bars represent standard errors of the mean (SEM) for three independent determinations. Non-ifx., non-infected macrophages; ifx, infected macrophages.