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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Circ Cardiovasc Genet. 2011 Aug 12;4(5):500–509. doi: 10.1161/CIRCGENETICS.111.960633

Figure 5.

Figure 5

FRET in Normal and Mutant Channels A. Diagram of FRET construct containing CFP fused to the C-terminus of hNav1.5 and YFP inserted into the Domain II-III linker at amino acid position 1022. B. Currents elicited from the FRET construct are similar to WT currents. C. FRETc values for YFP-1022 (n=43) were similar to YFP-1022+R558-40aa (n=35), YFP-1022+R558-20aa (n=39), YFP-1022/R282H+R558-40aa (n=43), YFP-1022/R282H+R558-20aa (n=46), and YFP-1022/R282H+R558-20aa+Brefeldin A (n=34). FRETc of YFP-1022/R282H (n= 45) alone, YFP-1022/R282H+H558-40aa (n=36), and YFP-1022/R282H+H558-20aa (n=42) were significantly smaller than WT. *P<0.05 compared to WT.