Table 2. The effects of acute psychological stress on CB1 receptor binding and signaling.
There was no effect of 30 min restraint stress on the maximal binding (Bmax) or the binding affinity (Kd) of the CB1 receptor, nor the maximal stimulation (Emax) or the EC50 of CB1 receptor-mediated 35S-GTPγS binding within the amygdala. Values denoted are means ± SEM. For all treatment conditions, n = 4.
Basal | Stress | |
---|---|---|
Amygdala | ||
Bmax (pmol/mg protein) | 0.11 +/- 0.01 | 0.17 +/- 0.05 |
Kd (nM) | 0.33 +/- 0.04 | 1.09 +/- 0.60 |
Emax (% baseline) | 188.0 +/- 6.3 | 181.7 +/- 10.1 |
EC50 (nM) | 81.0 +/- 8.5 | 127.6 +/- 22.5 |