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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Dev Dyn. 2011 Sep 26;240(11):2474–2481. doi: 10.1002/dvdy.22750

Fig. 1. Hgs−/− is a null mutation of Hgs gene.

Fig. 1

A–D: Whole mount in situ analysis of E8.5 embryos. Brachyury was expressed in the regressing primitive streak in WT (A, arrow). In Hgs−/− embryos, the expression was strongly decreased (B). Mox1, a somite marker (C), was not expressed in Hgs−/− embryos (D). Bar = 300 μm. E: Semi-quantitative RT-PCR analyses. The expressions of Hgs or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control of mRNA in WT or Hgs−/− embryos are shown. −RT is a negative control sample without reverse transcriptase.