Figure 5. Anti-Delta1 mAb upregulates Th2 cytokines.
(A) Donor specific Th1, Th2 and proinflammatory cytokine production of splenocytes from CD28KO recipients of BALB/c allografts were assessed 14 days after transplantation by Luminex assay. Anti-Delta1 treatment led to a significant increase in the production of Th2 cytokines (IL-4, IL-5), while it decreased the production of IL-2, IL-6 and IL-17. (B) The frequency of GrB-producing cells was also decreased on the Delta1 blockade group by ELISPOT. There was a trend towards lower IFN-γ but it did not reach statistical significance. Data are representative of 3 independent experiments and indicate the mean of triplicate results in each experiment (* p<0.05).