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Lentivirus expressing ShRNA for Smad2 or Smad3 specifically knocks down Smad expression in C8D1A cells. Four days after lentiviral infection of C8D1A cells, cells were lysed and Smad expression analyzed. Representative western blots showing (a) specific Smad2 knockdown and (b) specific Smad3 knockdown after lentiviral infection with Smad-specific shRNA. Quantification (c &d) of Smad immunoreactivity normalized to actin (mean ±SEM, n=3). (*p<0.05: t-test.).
