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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Dev Dyn. 2011 Sep 29;240(11):2495–2504. doi: 10.1002/dvdy.22754

Figure 1.

Figure 1

A newly identified allele of AXPC is predominantly expressed during early development.

A. Comparison of the sequence of the two alleles of AXPC surrounding the ATG start codon. MO-1 refers to a previously published construct (orange). MO-2 is a newly designed morpholino (green). Red box indicates the ATG start codon. Differences between the sequences denoted by an *. B. Western blot detection of both exogenous and endogenous expression of AXPC in lysates from embryos collected at the indicated stage. Embryos over-expressing AXPC (lane: AXPC) were collected at stage 12.5. C. Western blot of endogenous AXPC in embryo lysates at stage 12.5 injected with the indicated amount of control morpholino or AXPC morpholino. For co-injections, morpholino-1 and morpholino-2 were mixed in equal parts to give the indicated final amount. In B and C, AXPC was detected by western blotting with anti-AXPC antibody. Each lane represents the equivalent of 1 embryo.