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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Dev Dyn. 2011 Sep 29;240(11):2495–2504. doi: 10.1002/dvdy.22754

Figure 2.

Figure 2

Morpholino to the second AXPC allele causes developmental axial defects.

A–D. Embryos were injected on one side of 2-cell embryo with 10, 20, or 40ng (shown) of morpholino along with a Dextran-Ruby tracer. Embryos were cultured and observed at stages 22 (C,D) and 30 (A,B). Fluorescent Dextran-Ruby (red) images were superimposed onto brightfield images in C and D. E. Embryos were scored at stage 30 as a strong (>45°), mid/weak (0°<X<45°), or no (0°) phenotype. Anterior is up. F,G. Whole mount immunofluorescence on embryos bisected sagitally at stage 30, stained with anti-AXPC (green) and anti-fibronectin (red). Asterisks denote side of morpholino injection. Confocal images obtained from ventral aspect at 600X magnification. Scale bar = 50μm.