Skip to main content
. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Dev Dyn. 2011 Sep 29;240(11):2495–2504. doi: 10.1002/dvdy.22754

Figure 4.

Figure 4

AXPC does not mediate cell sorting.

A–C. In cell dispersal assays, embryos were injected into one cell at 32-cell stage with nuclear-GFP alone (A), GFP+PAPC (B) or GFP+AXPC (C) and cultured to stage 9. Scale bar = 0.5mm. D–F. Reaggregation assay performed using blastomeres isolated from excised animal caps expressing RFP, GFP, AXPC-mCherry, and PAPC+GFP. Cells were mixed as indicated and images were acquired once aggregates formed (5–6 hours). Scale Bar = 1mm. G,H. AXPC constructs have functional activity in perturbing notochordal morphogenesis. Sagittal cryosections of stage 12.5 embryos from injected dorsally at 2-cell stage, with 1ng RFP (I) or 1ng AXPC (J). C-cadherin, fibronectin (FN), dextran-TRITC (red). Confocal images taken at 200X magnification. Scale bar = 150μm.