Skip to main content
. Author manuscript; available in PMC: 2012 Aug 23.
Published in final edited form as: Oncogene. 2011 Jul 18;31(8):966–977. doi: 10.1038/onc.2011.291

Figure 4. Disruption of C20S multimerization abrogates suppression of PAX5 activity and stable chromatin binding.

Figure 4

(A) FRAP assay comparing YFP-PAX5-C20-ΔMD (gold squares, N=5, t1/2 ~ 2 s), YFP-PAX5-C20S-L421P (gray diamonds, N=5, t1/2 ~ 5 s), and YFP-PAX5-C20S (black circles, N=4, t1/2 ~ 200 s) at 24 h post-transfection in H1299 cells.

(B) YFP-PAX5-wt and the luciferase reporter were co-transfected alone, or with YFP-PAX5-C20S, YFP-PAX5-C20-MD, YFP-PAX5-C20-ΔMD, and YFP-PAX5-C20S-L421P, and luciferase activity assayed.