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. Author manuscript; available in PMC: 2012 Aug 23.
Published in final edited form as: Oncogene. 2011 Jul 18;31(8):966–977. doi: 10.1038/onc.2011.291

Figure 7. The C20S tetramerization domain suppresses the dominant negative activity of PAX5-C20S.

Figure 7

(A) Vectors for PAX5-wt, PAX5-C20S, and PAX5-C20SΔDBD and the luciferase reporter were transfected into 293T cells as indicated and luciferase assayed after 24 h. FRAP assays for (B) YFP-PAX5-ΔDBD-C20S (orange circles, N=3, t1/2 ~ 1 s), YFP-PAX5-C20S co-expressed with four-fold more CFP-PAX5-ΔDBD-C20S (YFP FRAP gray diamonds, N=7, t1/2 ~ 4 s), and YFP-PAX5-C20S (black circles, N=4, t1/2 ~ 200 s), and for (C) YFP-PAX5-ΔDBD-C20S (orange circles, N=3, t1/2 ~ 1 s), YFP-PAX5-ΔDBD-C20S and an equal amount of CFP-PAX5-C20S (gray circles, N=8, t1/2 ~ 25 s), and YFP-PAX5-C20S (black circles, N=4, t1/2 ~ 200 s). (D) PAX5-wtp53tetra was expressed with wt PAX5 and PAX5 reporter gene either with or without PAX5-C20SΔDBD and luciferase assayed.