FIG. 4.

USF1 and USF2 binding to the Fshr, Gata4, and Nr5a1 gene promoters in vivo during differentiation. Chromatin-protein complexes were immunoprecipitated with nonimmune serum (IgG) or antiserum against WT1, USF1, or USF2. The relative quantities of PCR amplified E-box regions from the Fshr (A), Gata4 (B), or Nr5a1 (C) promoters are shown for 5- and 11-day-old Sertoli cells. Recruitment to E-box regions was normalized to the relative quantity of complexes recruited by IgG for the same age cells (relative binding set equal to one). Results shown are the mean ± SEM of three experiments performed in triplicate. Experiments employing cells from 5- and 11-day-old rats were run concurrently. *P < 0.05 vs. the IgG value of the same age, ^##P < 0.05 vs. the same condition in 5-day-old rats.