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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Immunol. 2011 Sep 28;187(9):4890–4899. doi: 10.4049/jimmunol.1100381

FIGURE 4.

FIGURE 4

S. pneumoniae genomic DNA activates the AIM2 inflammasome. A: DNase I untreated and treated S. pneumoniae (Sp) genomic DNA was electrophoresed on a 0.8% agarose gel. B and C: Adherent PECs were transfected or not with Sp genomic DNA or DNase-treated Sp genomic DNA and incubated for 24h. B: Culture supernatants and cell lysates were subjected to Western blot analysis as described in Fig. 1. Filled and open triangles indicate MW markers of 47 kDa and 9 kDa, respectively. C: IL-18 in the supernatants was determined by ELISA. D: AIM2 knockdown or control macrophages were left untreated or transfected with Sp genomic DNA for 24 h and IL-18 in the supernatants was determined by ELISA. All of the experiments were repeated two times. The results are presented as the mean and SD of triplicate assays. Tests for statistical significance were performed by using one-way ANOVA followed by the Bonferroni’s test. *, p<0.05.