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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Nat Immunol. 2011 Oct 2;12(11):1086–1095. doi: 10.1038/ni.2106

Figure 5. CD8αα expression marks effector memory CD8αβ T cells in humans.

Figure 5

(a) Expression of CD8αα on polyclonal human naive (TN; CCR7+CD45RA+), recently activated effector-memory (TEMRA; CCR7–CD45RA+), effector-memory (TEM; CCR7–CD45RA–) and central-memory (TCM; CCR7+CD45RA–) CD8+ T cells was measured by TL-tetramer staining. The numbers indicate the percentage of TL-tetramerhi cells. Graph depicts pooled data ± s.e.m. on percentage of CD8αα expression on human peripheral blood CD8+ T cells (n = 9). The differences between TN and TEMRA, TEM or TCM were significant (P < 0.001, unpaired t-test). (b) TL-tetramer staining of human TEMRA CD8+ T cells is blocked by anti-CD8α but not anti-CD8β antibody. Data are representative of two independent experiments. (c) CMVpp65-specific CD8+ T cells display a TEM/TEMRA phenotype and persist at high frequency in humans. Data from two representative donors from a total of six persons are shown. The TL-tetramer staining was absent on naive CD8+ T cells and was blocked by an anti-CD8α.