FIG. 4.

High glucose treatment also results in failure to repair mechanically induced disruptions. C2C12 myoblasts were cultured for 2 or 8 weeks in 7.5 mmol/L glucose (N Gluc), 30 mmol/L glucose (H Gluc), or iso-osmolar control 30 mmol/L mannitol (Man) and then scraped from 96-well plates. A live/dead or MTT assay was then performed to assess cell survival. No significant differences were demonstrated after 2 weeks of treatment. However, 8 weeks of high glucose treatment decreased survival as determined by both assays. Data are presented as the mean ± SEM. *P < 0.01; n = 24 wells for each condition.