Skip to main content
. 2011 Oct 17;60(11):2985–2994. doi: 10.2337/db10-1333

FIG. 1.

FIG. 1.

Calpain activity and protein expression in STZ-injected and OVE26 mice. Enzymatic calpain activity was measured in sham and STZ-treated hearts (A) or wild-type (WT) and OVE26 hearts (B). C: The cleavage of α-spectrin (120/150 KD) was determined in sham and STZ-injected hearts by Western blot analysis. Top: A representative Western blot for cleaved fragments of α-spectrin from four of eight different hearts in each group; bottom: quantification of cleaved fragments of α-spectrin relative to GAPDH. D: Representative Western blot for calpain-1, calpain-2, and GAPDH proteins from four of eight hearts in each group (sham and STZ-induced hearts). D1 and D2 are the quantification of calpain-1 and calpain-2 protein relative to GAPDH. Data are mean ± SD; n = 8. KD, kDa. *P < 0.05 vs. sham or WT.