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. 2011 Oct 17;60(11):2985–2994. doi: 10.2337/db10-1333

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© 2011 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 2. — Characterization of mice with cardiac-specific Capn4 knockout. A: Representative RT-PCR for Cre and Capn4 mRNA from two of six mice in each group. Cre mRNA was detected in Capn4^−/− hearts (lanes 3 and 4). Levels of Capn4 mRNA were significantly reduced in Capn4^−/− hearts. In contrast, the Capn4 mRNA was not decreased in Capn4^−/− lungs. B: Capn4 mRNA was quantified by real-time RT-PCR in Capn4^+/+ and Capn4^−/− hearts (B1) and lung tissues (B2). C1 and C2 are the quantification of Capn4 mRNA in isolated cardiomyocytes (C1) and cardiac fibroblasts (C2) from Capn4^+/+ and Capn4^−/− mice. D: Representative casein zymography for calpain-1 and calpain-2 activities from two of six hearts and lungs, respectively, showing downregulation of calpain-1 and calpain-2 activities in Capn4^−/− hearts but not in lungs. Data are mean ± SD; n = 5–9. *P < 0.05 vs. ^+/+. OD, optical density.