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. 2011 Oct 17;60(11):3055–3066. doi: 10.2337/db11-0807

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© 2011 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 6. — High glucose–mediated mesangial cell growth inhibition and hypertrophy can be reversed by activation of Nrf2. A: Cell growth of HRMCs incubated in NG, HG, HG+tBHQ, HG+SF, or HG+CA DMEM media was monitored in real-time for 96 h (upper panel = average; lower panel = average with error). B: Cell death and proliferation were assessed by transferase-mediated dUTP nick-end labeling (TUNEL) assay (positive control is treated with cisplatin at 18 μmol/L for 24 h) or Ki67 immunolabeling. HG media induced cell growth inhibition, which was alleviated by coculture with an Nrf2 activator. C: Cell size of HRMCs incubated in NG, HG, HG+tBHQ, HG+SF, or HG+CA DMEM media for 96 h was measured by forward light scatter/flow cytometry. Incubation in HG media induced cellular hypertrophy that was reduced with an Nrf2 activator. D: Cell area is reported from GFP-transfected HRMCs incubated in NG, HG, HG+tBHQ, HG+SF, or HG+CA DMEM media for 48 h. Total cell area increased with HG conditions but was significantly reduced in the presence of an Nrf2 activator. Data in D are expressed as mean ± SD (n = 100). *P < 0.05 compared with NG group. #P < 0.05 Nrf2 activators compared with HG alone. FSC-H, forward scatter. SSC-H, side scatter. (A high-quality color representation of this figure is available in the online issue.)