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. 2011 Oct 17;60(11):2710–2719. doi: 10.2337/db11-0132

FIG. 1.

FIG. 1.

Effective deletion of Ucp2 specifically from pancreatic β-cells using a Cre-lox recombination strategy. A: Targeting construct for the Ucp2 gene shows the floxed region (flanked by loxP sites) and the Ucp2 allele after Cre recombinase excision. Homozygote loxUCP2 mice that express Cre undergo recombination of the DNA between loxP sites, deleting exons 3 and 4, which contains the start codon. B: Typical PCR results from mouse genotyping for loxUCP2 (floxing) and Cre expression. C: Quantitative PCR results show reduced Ucp2 mRNA expression in isolated UCP2BKO islets compared with RIPCre islets. Ucp2 mRNA expression was calculated as a percentage of β-actin. The error bar shows the SEM. D: Standard PCR of various tissues shows full-length UCP2 transcripts and truncated UCP2 (Δ) where exons 3 and 4 have been removed by Cre recombinase activity. Wt, wild type. E, Two left panels: Dispersed pancreatic islets isolated from RIPCre and UCP2BKO mice immunostained for UCP2 (red) and insulin (green). Two right panels: UCP2BKO dispersed islet cells stained for Cre (red) and insulin (green) show nuclear localization of Cre recombinase. The yellow coloring represents colocalization of red and green fluorescence. n = 5–7. *P < 0.05. (A high-quality digital representation of this figure is available in the online issue.)