FIG. 5.

Both Tregs and diabetogenic BDC2.5 clonotypic CD4 T cells expand within PLNs of NOD mice depleted of FO B cells by anti-CD20 treatment. Beginning at 4 weeks of age, NOD females received two injections at a 21-day interval of the CD20-specific or control antibody and at 4–7 days after the second treatment, were assessed for numbers of (A) total CD4 T cells, (B) BDC2.5 clonotypic cells by specific tetramer staining, and (C) Tregs within PLNs. Noted statistical significance values were determined by ANOVA analyses. Aby, antibody.