Table 2. Methylation of H19DMR in embryos, in which parental allele discrimination could be carried out and in sperms.

No of embryo	Embryo type	♂ Allele % methylation	♀ Allele % methylation	Sperm % methylation
4.1	CM	68.75 (99/144)*	8.34 (3/36)*	97.68 (211/216)
4.3	CM	15.07 (19/145)*	0 (0/36)	97.68 (211/216)
4.5	CM	79.17 (57/72)	1.11 (1/90)	97.68 (211/216)
5.4	P	51.85 (28/54)*	0 (0/36)	96.66 (261/270)
26.2	BC	77.78 (126/162)*	0 (0/18)	100 (36/36)
26.4	BC	94.11 (170/180)	5.56 (1/17)	100 (36/36)
30.1	BC	78.70 (85/108)	2.56 (9/117)*	94.07 (254/270)
30.2	P	75.93 (123/162)*	0.93 (1/108)	94.07 (254/270)
30.3	CM	75.39 (95/126)*	7.40 (4/50)*	94.07 (254/270)
30.4	CM	90.48 (114/126)	5.55 (5/90)*	94.07 (254/270)
31.1	CM	69.44 (75/108)*	0 (0/90)	93.98 (203/216)
33.3	P	54.17 (39/72)*	15.87 (20/126)*	93.70 (253/270)
46	CM	78.70 (85/108)	0 (0/72)	95.55 (258/270)
50.1	M	81.11 (73/90))	1.11 (1/90)	90.87 (229/252)
52.1	CM	84.44 (76/90)	6.35 (8/126)*	98.01 (247/252)
52.2	CM	88.89 (48/54)	20.14 (29/144)*	98.01 (247/252)
52.5	P	80.56 (58/72)	3.96 (5/126)*	98.01 (247/252)
52.6	BC	81.75 (103/126)	1.39 (2/144)	98.01 (247/252)
56.2	BC	93.33 (84/90)	0 (0/90)	94.44 (255/270)
56.3	P	88.89 (80/90)	2.08 (3/144)	94.44 (255/270)
56.4	CM	93.21 (151/162)	2.22 (2/90)	94.44 (255/270)
2	Control	89.50 (145/162)	0 (0/198)
5	Control	83.33 (90/108)	0 (0/162)
6	Control	84.92 (214/252)	0 (0/198)
8	Control	85.18 (184/216)	1.66 (3/177)
Control sperm
a				96.49 (275/285)
b				93.65 (236/252)

Abbreviations: M, morula; CM, compacted morula; BC, abnormal BC blastocysts; P, pre-blastocysts; control, high-graded blastocysts, suitable for transfer. a, sperm from a fertile man; b, pool of sperms from five fertile men.

^*P≤0.005.