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. 2011 Oct 17;195(2):203–215. doi: 10.1083/jcb.201102115

Figure 2.

Figure 2.

Btn1 localizes to the Golgi in a manner dependent on Btn2. (A) Btn1 does not colocalize with FM4-64–labeled compartments. (top) WT cells expressing GFP-tagged BTN1 from its genomic locus under the control of a GAL promoter (GAL integrated [GAL int]) were grown on galactose-containing medium and were labeled with FM4-64. (middle) WT cells expressing GFP-BTN1 constitutively from a single-copy plasmid (CEN plasmid) were grown on glucose-containing medium and were labeled with FM4-64. (bottom) WT cells expressing GFP-BTN1 from its genomic locus and under the control of the BTN1 promoter were grown on glucose-containing medium and were labeled with FM4-64. (B) Btn1 colocalizes with Yif1. WT cells expressing GFP-BTN1 from its genomic locus under the control of a GAL promoter and either RFP-YIF1 from a single-copy plasmid (top row) or SEC7-DsRed from a chromosomal integration (bottom row) were grown on galactose-containing medium and visualized. (C) Btn1 and Btn2 do not colocalize. WT cells expressing GFP-BTN1 from its chromosomal locus under the control of a GAL promoter and BTN2-RFP from a single-copy plasmid are shown; cells were grown on galactose-containing medium. (D) Btn1 is mislocalized to the vacuole in btn2Δ cells. btn2Δ cells expressing GFP-BTN1 from a single-copy plasmid were grown on glucose-containing medium and were labeled with FM4-64. Bars, 1 µm.