Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Oct 17;195(2):193–201. doi: 10.1083/jcb.201104076

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Yi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Rapid dispersal of organelles by acute dynein inhibition. Lysos/LEs rapidly redistribute to the cell periphery by 74.1 Ab microinjection in COS-7 cells within 10 min after injection. (A) Uninjected, IgG, and 74.1 Ab–injected cells are shown at 0 and 10 min after injection. The rightmost panels are time projection images at 1, 5, and 10 min after injection. Kymographs of the indicated regions (dotted rectangles) are shown in the bottom row. Only the 74.1 Ab–injected cell showed en masse lysosome dispersal as shown in the kymograph as plus-biased motion. (B) GFP-NPC1, Rab5-GFP (early endosomes), N-acetylglucosaminyltransferase–GFP (Golgi), and adenovirus Alexa Fluor 546 were dispersed. Dotted circles denote dispersed particles. (C) LysoTracker-positive particles were also dispersed by 70.1 Ab, NudE/L antibody, and the purified CC1 fragment of the p150^Glued dynactin protein. (D) N-terminal p150^-Glued dynactin antibody, function-blocking LIS1 antibody, and DN LIS1 protein did not affect overall lysosome distribution. All images were taken immediately after injection (0 min) and after 10 min postinjection unless specified otherwise. Bars, 10 µm.