Table I.

ORFs in diverse eukaryotes that possess conserved nuclear membrane protein domains

Species	SUN domain (PF07738)	LUMA (DUF1625)	LEM domain (PF03020)	KASH (PF10541)	Nurim
Homo sapiens	NP_001165415, NP_079430, NP_001186508, NP_056189, NP_689995, NP_542406, NP_003107	NP_077310	NP_001161086, NP_055134, NP_001001552, NP_001185979, NP_851853, NP_055929	NP_892006, NP_149062, NP_878914, NP_689805, NP_878918, NP_055995, NP_878917,	NP_009174
Drosophila melanogaster	NP_610240, ABQ08586	NP_648162	ABY20424, NP_609281, NP_610099	NP_001188695, AAO42670, NP_001188694, NP_001188696, AAN60444, NP_001188697, AAM11072, AAN35199, NP_001097463, NP_001097462, NP_001036576, NP_523873	NP_650646
Caenorhabditis elegans	NP_001024707, NP_506281		NP_490907, NP_496944, NP_492539	NP_491353
Nematostella vectensis	XP_001632145	XP_001622563, XP_001626986	XP_001638261
Monosiga brevicollis	XP_001747062
Capsaspora owczarzaki	EFW43369	EFW40407			EFW41164
Saccharomyces cerevisiae	NP_012515
Neurospora crassa
Schizosaccharomyces pombe	NP_595947
Dictyostelium discoideum	XP_644924				XP_636885
Entamoeba histolytica
Arabidopsis thaliana	NP_196118, NP_566380
Chlamydomonas reinhardtii
Thallasiosira pseudonana	XP_002288849	XP_002296785
Phytophthora ramorum	XP_002997929
Paramecium tetraurelia		XP_001461995, XP_001425037, XP_001441689, XP_001453417, XP_001444222, XP_001425204, XP_001428586, XP_001425039
Plasmodium falciparum	XP_001350555
Trypanosoma brucei
Naegleria gruberi	XP_002675222	XP_002683403
Trichomonas vaginalis
Giardia intestinalis	EES98800

Search results for selected nuclear protein ORFs in representatives of five eukaryotic supergroup lineages, listing genes or ORFs homologous to each queried polypeptide that were detected in completed genomes of representative supergroup lineages (Opisthokonts, Amoebozoa, Archaeplastida, Chromalveolates, and Excavates) in GenBank using reciprocal BLAST with e-values > 0.01. This method detected ORFs representing four of the seven known human LEM domain genes, and is therefore quite stringent. Multiple ORFs, for example LUMA-related ORFs in Paramecium, were not annotated so the number of encoding gene(s) remains to be determined.