Fig. 2.

Antibodies against carbamylated proteins are present in sera of RA patients. The reactivity of IgG (A and B) and IgA (D and E) from sera of healthy controls (NHS) or RA patients (RA) to wells coated with nonmodified FCS (FCS) or carbamylated FCS (Ca-FCS) is depicted. Data expressed as absorbance at 415 nm. (C and F) Absorbance units of FCS were subtracted from the absorbance units of Ca-FCS, representing the specific anticarbamylated protein response. ***P < 0.0001 for a t test comparing NHS and RA.