Fig. 3.

Nyv1p-CCIIM is active in chaperone-mediated fusion reactions. Reactions contained 1 mM MgCl₂, an ATP-regenerating system, RPLs bearing Nyv1p-CCIIM (×2: protein/lipid ratio = 2:1,000; ×1: 1:1,000; ×0.5: 0.5:1,000; ×0.25: 0.25:1,000) or Nyv1p at corresponding levels, 2Q-SNARE RPLs (protein/lipid ratio = 1:1,000), Vam7p (600 nM), HOPS (40 nM), Sec17p (0.68 μM), and Sec18p (0.24 μM). In a control reaction, the 2Q-SNARE RPLs were omitted. The maximal rates of lipid mixing were calculated based on four independent experiments (Materials and Methods). Error bars represent SDs. A typical experiment is shown in Fig. S1.