Fig. 6.

Knockdown of MYC in recurring lymphomas causes cell cycle arrest and increased apoptosis. (A) Levels of MYC protein in parental and recurring tumors lines infected with either control (CTRL) shRNA or MYC shRNA, which specifically targets human MYC. (B) Time course of the percentage of GFP⁺ shRNA infected populations after MYC knockdown. Values are normalized to the initial percentage of GFP⁺ cells after infection. (C) Apoptosis in control or MYC shRNA infected cells, as measured by Annexin-V staining. (D) Western blot on shRNA infected cells for expression of p27KIP1 and Bim. Experiments were repeated a minimum of three times, and representative experiments are shown. *P < 0.01, **P < 0.001, paired t test. (E) Model of tumor recurrence and continued dependence of recurring tumors on MYC.