Table 1.
Strains used in this study and averaging details*
| Name | Strain(s) | Ref. | Structure visualized | Averaged repeats | Resolution, nm | Shown in figures |
| Chlamydomonas | ||||||
| pWT | pf2-4::PF2-GFP† | 14 | DMTs (DMT1–9) | 720 | 3.4 | 1 A, B, and D–F; 2; 3 A–F; 4 C–J; S1; S2 A′–F′; S3 F and G |
| DMTs without beak-MIP (DMT2, DMT3, DMT4, and DMT7–9) | 590 | 3.4 | 4 A and B | |||
| DMTs with beak-MIP (DMT1, DMT5, and DMT6) | 210 | 3.9 | 4 K–N | |||
| drc Mutants‡ | pf3 (CC-1026) | 14, 22 | Inner AB-junction without hole (DMT1–9) | 1,980 | 3.3 | 3 G and H |
| ida6 (CC-3090) | 23 | |||||
| 4D6 (CaM-IP2 amiRNAi) | 24 | |||||
| S. purpuratus (sea urchin) | ||||||
| Flagella | DMTs (DMT1–9) | 470 | 3.6 | S2 A′′–F′′, G, H, and J | ||
| Isolated DMT | Inner AB-junction (DMT1–9) | 118 | 3.8 | S2I | ||
*Some Chlamydomonas data were refined from data originally published by Nicastro et al. (10) and Heuser et al. (14) and were combined with previously undescribed data to improve resolution.
†A pseudo-WT (pWT) strain was obtained by transformation of the pf2 mutant with the WT PF2-GFP gene, which rescues the structural defects in the pf2 mutant (14).
‡Axonemal repeats from three different mutants with defects in the assembly of the N-DRC base plate were averaged together.